The BioPace Long Range PCR Kit (100) is helpful for specific long-range PCR for up to 30 kb that helps in streamlining the workflow. Clear-sequencing results are produced due to a polymerase-proofreader blend that has 10X higher fidelity than Taq offering better specificity as well as sensitivity.
BioPace™ Long-Range PCR Kit
Price range: $205.00 through $861.00
Purchase this item and get Product Description
The BioPace™ Long Range PCR Kit (100) is a powerful tool for specific long-range PCR, enabling streamlined workflows with amplicons of up to 30 kb. It ensures clear sequencing results through a polymerase-proofreader blend with 10X higher fidelity than Taq, enhancing specificity and sensitivity. The kit is designed for various applications, including immunology, cancer, and microbial workflows, providing exceptional speed and moderate multiplexing capability. Visual pipetting control and tracking dyes monitor successful procedures, while the 4X-concentrated master mix format allows higher sample input volumes. With automation readiness, stability at room temperature, and a hot-start mechanism, the kit facilitates efficient and reliable PCR.
The AVBlend™ enhances the amplification of challenging targets, and the sophisticated buffer composition supports long-range amplification with a combined 30 seconds/kb annealing-extension step. High yields are achieved from minimal template amounts, improving screening workflows. The uracil proofreader offers high template affinity and 10X higher fidelity, while the master mix formulation allows moderate multiplexing of up to 6 targets. Integrated visual pipetting and gel loading dyes enhance efficiency in genotyping and genetic testing workflows, making the BioPace LongRange PCR Kit a versatile and comprehensive solution for long amplicon sequencing made possible by the OptiClean™ (nucleic acid-depleted) Guide Mix and the blue template guide in a specialized mix of polymerases and PCR buffer. Now amplify large full-length amplicons with the ease of a standard PCR.
Kit Contents
For 100 PCR reactions:
● 4X BioPace™ Long Range PCR Master Mix (1 x 0.5 mL)
● 25X OptiClean™ Template Guide (1 x 0.05 mL)
● 125X Guide Master Mix
● 5X AvBlend™ (1 X 2 mL)
● 1 x 1.9 ml Nuclease-Free Water
Technical Parameters
10X higher fidelity than standard Taq Polymerase 6-Plex Multiplex
Automation Ready
Amplification of 2kb/minute
Target range of 1-9kb
Applications
Optimal for the following applications:
● Long Range amplicons
● 16S, 23S or ITS from fungal, bacterial, oncogenic or HLA sources
Product FAQs
How does the 4X-concentrated master mix format contribute to the kit's performance?
The performance of this kit is driven by the master mix supplied at a 4X concentration to allow ease of use with a wide range of template concentrations and minimal pipetting steps and need of optimization during reaction setup.
What is the maximum amplicon size achievable with the BioPace Long Range PCR Kit?
A 9 kilobase amplicon size can be achieved with the BioPace Long Range PCR kit.
How does the polymerase-proofreader blend in the kit enhance sequencing results?
The proofreading activity of polymerases can prove critical for the success of large amplicon size PCR reactions. Errors during extension of large amplicons might lead to non-specific results. The kit blend is designed with a polymerase with inherent 5’-3’ proofreading activity to avoid any such occurrences.
How do the visual pipetting control and tracking dyes aid in reaction setup?
The blue dye in the buffer is added for visual tracking of a user to monitor where the reaction mix has already been pipetted. PCR can be a tricky reaction to set up with colorless mix components as they do not allow the user to monitor where the mix has already been added. This highly avoids the chance of multiple pipetting and cross contamination, improving yields and accelerating optimization.
| Reactions | 100, 500 |
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